Stochastic nucleosome positioning in a yeast chromatin region is not dependent on histone H1

To gain a better understanding of the function of the yeast histone HI, its role in nucleosome positioning was studied. With this objective in mind, w e analyzed a chromatin region of the yeast Chromosome (Chr) IX, in which th ere are two closely packed open reading frames (ORFs), POT1 and YIL161w. Th is locus shows a regular ladder of 13 stochastically positioned nucleosomes , which is unaffected by the absence of the HHO1 gene. This suggests that h istone H1 has no effect on nucleosome positioning in yeast.