To gain a better understanding of the function of the yeast histone HI, its
role in nucleosome positioning was studied. With this objective in mind, w
e analyzed a chromatin region of the yeast Chromosome (Chr) IX, in which th
ere are two closely packed open reading frames (ORFs), POT1 and YIL161w. Th
is locus shows a regular ladder of 13 stochastically positioned nucleosomes
, which is unaffected by the absence of the HHO1 gene. This suggests that h
istone H1 has no effect on nucleosome positioning in yeast.