ISOLATION AND PARTIAL CHARACTERIZATION OF A FATTY-ACID-BINDING PROTEIN FROM ASCARI-SUUM REPRODUCTIVE TISSUE

Authors
TIMANOVA AM MARTI T WALTER RD BANKOV IY
Citation
Am. Timanova et al., ISOLATION AND PARTIAL CHARACTERIZATION OF A FATTY-ACID-BINDING PROTEIN FROM ASCARI-SUUM REPRODUCTIVE TISSUE, Parasitology research, 83(5), 1997, pp. 518-521
Citations number
19
Categorie Soggetti
Parasitiology
Journal title
Parasitology research → ACNP
ISSN journal
09320113
Volume
83
Issue
5
Year of publication
1997
Pages
518 - 521
Database
ISI
SICI code
0932-0113(1997)83:5<518:IAPCOA>2.0.ZU;2-8
Abstract
A 12-kDa fatty-acid-binding protein was purified to homogeneity from A scaris suum reproductive tissue as confirmed by sodium dodecyl sulfate -polyacrylamide gel electrophoresis. N-terminal amino-acid-sequence an alysis of the protein revealed its identity with the ABA-1 allergen pr otein isolated from A. suum pseudocoelomic fluid. Fatty-acid binding b y the protein from A. suum reproductive tissue was investigated using the Lipidex 1000 assay, which revealed the presence of a single class of fatty-acid-binding sites with an apparent dissociation constant for palmitate of about 0.8 mu M.