IMMUNOHISTOCHEMICAL CHARACTERIZATION OF A NEW MONOCLONAL-ANTIBODY REACTIVE WITH DENGUE VIRUS-INFECTED CELLS IN FROZEN TISSUE USING IMMUNOPEROXIDASE TECHNIQUE

This paper presents a novel monoclonal antibody shown to react with cy toplasmic antigens in various dengue infected human frozen organs from autopsy and necropsy specimens. Strong reactivity was found in hemato poietic cells, including immunoblasts, lymphocytes, plasma cells and m acrophages of spleen, lymph node, lung, kidney and stomach. Strikingly , strong positivity was demonstrated in cerebral cortex neurones, Purk inje cells, choroid plexus and blood vessels in addition to astrocytes and microglia. Neurotropism of the virus could explain the meningitis , encephalitis, mononeuropathy and polyneuropathy observed by direct t oxicity, but noted especially after an activation of mononuclear phago cytes and amplification of the immune response with subsequent vascula r inflammation and formation of immune complexes.