HLA-G and classical HLA class I transcripts in various components of the adult human eye

HLA-G transcripts have previously been detected in the ocular cell line HS7 38, in foetal eyes and in global ocular extract. Using RT-PCR and hybridiza tion, we searched for HLA-G and classical HLA class I transcripts in the co rneas of eye donors (n = 6) and in various components of eyes collected at time of surgery: lens (n = 3), iris (n = 2), vitreous body (n = 2) and reti na (n = 2). Whereas HLA class I mRNA was abundant in the ocular tissues of all donors, we could not find HLA-G transcript in any ocular tissues obtain ed from subjects in vivo. The absence of HLA-G in adult eye cornea limits i ts interest as a possible target in corneal transplantation, Classical HLA class I transcripts were abundant in the cornea and other components of the eye. These results highlight the role of classical HLA class I antigen in eye immunity and corneal transplantation.