A cell-counting factor regulating structure size in Dictyostelium

Developing Dictyosfelium cells form large aggregation streams that break up into groups of 0.2 x 10(5) to 1 x 10(5) cells. Each group then becomes a f ruiting body. sm1A cells oversecrete an unknown factor that causes aggregat ion streams to break up into groups of similar to 5 x 10(3) cells and thus form very small fruiting bodies. We have purified the counting factor and f ind that it behaves as a complex of polypeptides with an effective molecula r mass of 450 kD. One of the polypeptides is a 40-kD hydrophilic protein we have named countin. In transformants with a disrupted countin gene, there is no detectable secretion of counting factor, and the aggregation streams do not break up, resulting in huge (up to 2 x 10(5) cell) fruiting bodies.