TGF-beta receptor expression in human odontoblasts and pulpal cells

Transforming growth factor-beta (TGF-beta) isoform expression by odontoblas ts leads to their sequestration within the dentine matrix, from where they may be released during caries and participate in the reparative processes. Two receptor types for TGF-beta have been implicated in TGF-beta induced si gnalling. The aim of this study was to characterise immunohistochemically t he expression of these receptors in sound and carious human teeth to facili tate our understanding of the ability of these cells to respond to TGF-beta stimulation. Sound and carious human teeth were routinely processed and pa raffin sections stained for TGF-beta receptors I and II, using the StrAviGe n immunoperoxidase method. Strong specific staining for both receptor types was observed in the odontoblasts of healthy teeth with the greatest intens ity seen with receptor I. Staining of weaker intensity was also observed fo r both receptors in the underlying cell rich area and pulp core. Similar pa tterns of staining were observed within carious tissues. We conclude that o dontoblasts and other cells of the pulp of mature human molar teeth show th e presence of both TGF-beta receptors I and II in health and disease with o dontoblasts showing the strongest expression. Such findings may be importan t in the response of these cells to tissue injury.