A UNIFIED PROCEDURE FOR THE CORRECTION OF LIQUID-JUNCTION POTENTIALS IN PATCH-CLAMP EXPERIMENTS ON ENDO-MEMBRANES AND PLASMA-MEMBRANES

Correct determination of absolute values and polarities of liquid junc tion potentials (LJPs) is essential in patch clamp experiments for the estimation of ionic selectivities and activation voltages of ion chan nels. A simple approach for the correction of membrane voltages for LJ Ps has been developed. The method covers all combinations of LJPs betw een the solutions of bath, patch pipette and reference salt bridge and is applicable to all patch configurations on plasma membranes and end omembranes.