Purification and ligand binding of EmrR, a regulator of a multidrug transporter

Authors
Brooun, A Tomashek, JJ Lewis, K
Citation
A. Brooun et al., Purification and ligand binding of EmrR, a regulator of a multidrug transporter, J BACT, 181(16), 1999, pp. 5131-5133
Citations number
21
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
16
Year of publication
1999
Pages
5131 - 5133
Database
ISI
SICI code
0021-9193(199908)181:16<5131:PALBOE>2.0.ZU;2-M
Abstract
EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pu mp-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbony l cyanide p- (trifluoro-methoxy) phenylhydrazone-with affinities in the mic romolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR.