Improved methods for encoding and decoding dialkylamine-encoded combinatorial libraries

Authors
Fitch, WL Baer, TA Chen, WW Holden, F Holmes, CP Maclean, D Shah, NH Sullivan, E Tang, M Waybourn, P Fischer, SM Miller, CA Synder, LR
Citation
Wl. Fitch et al., Improved methods for encoding and decoding dialkylamine-encoded combinatorial libraries, J COMB CHEM, 1(3), 1999, pp. 188-194
Citations number
12
Categorie Soggetti
Chemistry & Analysis",Chemistry
Journal title
JOURNAL OF COMBINATORIAL CHEMISTRY
ISSN journal
15204766 → ACNP
Volume
1
Issue
3
Year of publication
1999
Pages
188 - 194
Database
ISI
SICI code
1520-4766(199905/06)1:3<188:IMFEAD>2.0.ZU;2-S
Abstract
Optimized methods for utilizing and analyzing dialkylamine tags in encoded combinatorial chemistry are reported. A previously described LC/fluorescenc e method has been shortened from over 1 h to 6 min. A novel ion exchange LC /MS method for decoding has been developed which is both fast and rugged. T he use of quantitative encoding schemes is described. These new techniques have allowed a simplified, automated sample preparation scheme, and over 30 0 encoded bead samples can be read in a day.