Improved detection systems for TT virus reveal high prevalence in humans, non-human primates and farm animals

TT virus is a newly described agent infecting humans. Initially isolated fr om a patient (initials T, T,) with unexplained hepatitis, the virus has sin ce been found in both normal and diseased individuals. In the present study , we utilized genomic-length sequences from distinct genotypes of TT virus to design PCR-based assays using conserved oligonucleotide primers from thr ee independent regions of the virus genome. Each of the three assays was fo und to be superior to the PCR-based assays previously published. The most s ensitive of the new assays was utilized to demonstrate the prevalence of TT virus to be at least 34.1% in volunteer blood donors, 39.6% in commercial blood donors, 59.6% in non-A-GB hepatitis cases, 81.7% in injectable drug u sers and 95.9% in haemophiliacs. In an attempt to identify a possible sourc e of human infection, we found TT virus sequences to be present in 19% of c hickens, 20% of pigs, 25% of cows and 30% of sheep. Sequence determination and phylogenetic analyses demonstrated that isolates from farm animals were not genetically distinct from those found in humans. This study clearly de monstrates that previously reported PCR assays dramatically underestimate t he true prevalence of TT virus within the human population. Due to the high rate of infection in both blood donors and those with non-A-GB hepatitis, these results question the causal role of TT virus in cases of unexplained hepatitis. Further, it is possible that domesticated farm animals serve as a source of human infection.