Gl. Cote et al., Some structural features of an insoluble alpha-D-glucan from a mutant strain of Leuconostoc mesenteroides NRRL B-1355, J IND MIC B, 23(1), 1999, pp. 656-660
Citations number
22
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Leuconostoc mesenteroides strain NRRL B-1355 produces two soluble extracell
ular alpha-D-glucans from sucrose: alternan and dextran, An unusual mutant
strain derived from NRRL B-1355 has recently been isolated which produces p
ractically no soluble polysaccharide, but significant amounts of an insolub
le D-glucan, Methylation analysis shows it contains linear (1-->3) and (1--
>6) linkages as well as (1-->2) and (1-->3) branch linkages. The insoluble
glucan was partially digestible by endodextranase, giving rise to a series
of oligosaccharides, a high-molecular weight soluble fraction and an insolu
ble residue. Treatment of the soluble dextranase-limit fraction with an alp
ha(1-->2) debranching enzyme led to further dextranase susceptibility. Meth
ylation, FTIR and NMR analyses of the dextranase-treated fractions indicate
a non-uniform structure with domains bearing similarities to L. mesenteroi
des strain NRRL B-1299 dextran and to insoluble streptococcal D-glucans.