Pullulan production from deproteinized whey by Aureobasidium pullulans

The production of pullulan from deproteinized whey by Aureobasidium pulluia ns P56 was investigated using an adaptation technique and a mixed culture s ystem, The adaptation of A. pullulans and the mixed cultures of A, pullulan s and/or Lactobacillus brevis X20, Debaryomyces hansenii 194 and Aspergillu s niger did not increase the production of polysaccharide, Enzymic hydrolys is of lactose in deproteinized whey gave a higher polysaccharide concentrat ion and polysaccharide yield than acidic hydrolysed lactose, Maximum polysa ccharide concentration (11.0 +/- 0.5 g L-1), biomass dry weight (10.5 +/- 0 .4 g L-1), polysaccharide yield (47.2 +/- 1.8%) and sugar utilization (93.2 +/- 2.8%) were achieved using enzyme-hydrolysed whey (pH 6.5) containing 2 5 g L-1 lactose and supplemented with K2HPO4 0.5%, L-glutamic acid 1%, oliv e oil 2.5%, and Tween 80 0.5%, In this case the pullulan content of the cru de polysaccharide was 40%.