Parvovirus B19 infection induces apoptosis of erythroid cells in vitro andin vivo

Objectives: intrauterine parvovirus B19 infection is related to non-immune hydrops fetalis, the pathogenesis of which is based on the strict tropism o f B19 for erythroid precursor cells and the massive destruction of the infe cted erythroid cells, although the mechanism of B19-induced cytotoxicity ha s not been studied in detail, The purpose of this study is to provide empir ical evidence that B19 induces apoptosis of erythroid cells both in vitro a nd in vivo. Methods: we analysed culture cells infected in vitro by B19 and tissues of nine cases of hydrops fetalis caused by B19 intrauterine infection by histo logical and biological methods, Results: cells infected in vitro by B19 showed nuclear changes characterist ic of apoptosis by light microscopic examination and DNA extracted from the infected cells was fragmented. Electron microscopic examination showed the nuclei of infected cells contained crescent-shaped clumps of heterochromat in with increased density and double staining with anti-B19 antibody and te rminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphat e-digoxigenin nick-end labeling (TUNEL) confirmed apoptosis of individual c ells. Tissues of cases of hydrops fetalis caused by B19 contained erythroid cells with nuclear inclusions and characteristic nuclear changes of apopto sis by light microscopy The double-staining confirmed apoptosis of erythroi d cells in the tissues. Immunohistochemical analysis with antibodies agains t cellular factors involved in apoptosis showed that caspase3, p53 and p21 were positive in infected cells. Conclusions: these data indicate that B19 infection causes apoptosis of inf ected cells and cellular factors, caspase3, p53 and p21, may play important roles in the apoptotic pathway induced by B19.