Measurement of homonuclear (2)J-couplings from spin-state selective double-/zero-quantum two-dimensional NMR spectra

Authors
Permi, P Heikkinen, S Kilpelainen, I Annila, A
Citation
P. Permi et al., Measurement of homonuclear (2)J-couplings from spin-state selective double-/zero-quantum two-dimensional NMR spectra, J MAGN RES, 139(2), 1999, pp. 273-280
Citations number
28
Categorie Soggetti
Chemistry & Analysis","Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MAGNETIC RESONANCE
ISSN journal
10907807 → ACNP
Volume
139
Issue
2
Year of publication
1999
Pages
273 - 280
Database
ISI
SICI code
1090-7807(199908)139:2<273:MOH(FS>2.0.ZU;2-8
Abstract
H-1-detected two-dimensional double-/zero-quantum experiments are described for measurement of homonuclear (2)J(HH)-couplings of NH2 or CH2 groups in proteins. These experiments utilize multiple-quantum coherence for determin ation of the size and the absolute sign of the geminal scalar and dipolar c ouplings in the presence of broad lines. Spectra are simplified by gradient selection and spin-state selective filters. (C) 1999 Academic Press.