Dissociation between the state of leukocyte adhesiveness/aggregation in the peripheral blood and the availability of the CD11B/CD18 and CD62L antigens on the surface of the cells in patients with stress

We adopted whole blood flow cytometry and direct labeling of the CD11b/CD18 and the CD62L antigens to study the relationship between their expression on the surface of peripheral leukocytes and the state of leukocyte adhesive ness/aggregation (LAA) as revealed by the leukergy test. We examined patien ts with infection/inflammation, acute stress and controls. The mean +/- S.D . channel fluorescence intensity of CD11b/CD18 antigen did not differ betwe en patients with infection/ inflammation (173+/-78) and controls (167+/-72) . However, a significant (p<0.0001) difference between these groups was not ed regarding LAA state. There was a significant (p=0.04) reduction in CD11b /CD18 in stress (135+/-60) and a significant (p<0.001) increment in LAA. In both study groups, there was a significant reduction in CD62L. Patients we re divided into those with CD11b/CD18 above and below the control's average . No correlation was found between the antigens and LAA. We assume that LAA in patients with stress state is CD11b/CD18 and CD62L independent.