Secondary metabolites in transformed root cultures of Campanula glomerata

The transformed roots of Campanula glomerata L. var. dahurica Fish., induce d by infection with Agrobacterium rhizogenes MAFF 03-01724, produced the an thocyanins cyanidin 3-glucoside and cyanidin 3-rutinoside, a flavonoid ruti n and a phenylpropanoid glucoside coniferin, together with the polyacetylen e constituents lobetyol, lobetyolin and lobetyolinin. Although illumination was inhibiting for root proliferation, it was an essential factor for the accumulation of anthocyanin and flavonoid in the tissues. Addition of benzy ladenine (BA) to the medium also promoted the accumulation of phenolics (an thocyanins, rutin and coniferin) in the roots. Polyacetylene production in the roots was not greatly affected by either illumination or BA treatment.