Drought stress response in enzymatic activities of cowpea nodules

The response to drought stress of several nodule enzymes in cowpea (Vigna u nguiculata (L.) Walp.) N. IPA 204 at different stages of N-2 fixation devel opment, as well as their changes during the period of stress recovery are d escribed. Stress was applied continuously by the control of water potential through a porous cup. Stress applied during the P-2 stage (15 -30 d) inter fered most. The recovery in nodule metabolic activity at the pi stage (0-15 d) was higher than at other stages. There was a slight reduction in PEPC a ctivity with increased stress. NADH-GOGAT activity was the enzyme most sens itive to drought stress and most closely associated with N(2)ase (ARA in cl osed systems). The coupling between GS and NADH-GOGAT activities was lost a s drought stress progressed. GDH activity remained not only insensitive to the stress but its activity even increased, indicating that cowpea nodules maintain sufficient activity of some assimilatory enzymes under drought str ess.