By use of chlorambucil, we have generated a mouse mutation called scraggly
(sgl) that exhibits skin and hair defects. Homozygous mutant mice exhibit h
air loss, skin defects, and abnormalities in sebaceous lipid composition. W
e have constructed a high-resolution genetic map of mouse Chromosome (Chr)
19 that links this mutation to the anonymous DNA marker D19Umil. An additio
nal cross, (BALB/c x CAST/Ei) F-1 x BALB/c, was used to map markers around
this mutation as well as to map the potential candidate genes, Fgf8 and Cyp
17. Allelism tests between sgl and asebia (ab), another hair loss mutation
on mouse Chr 19, showed that these genes were separate and distinct.