Construction and optimization of a dog whole-genome radiation hybrid panel

A dog whole-genome radiation hybrid (WGRH) panel including 126 clones was c onstructed by fusing dog fibroblasts irradiated at 5000 rads with thymidine kinase-deficient hamster cells. The average retention frequency of the pan el designated as RHDF5000 is 21%, and its resolution power is estimated at 600 kb. The data provided by typing 400 markers were used to estimate linka ge power changes subsequent to panel reduction. These changes were analyzed by recomputing typing data from five reduced panels. From these simulation s, the parameters allowing investigation of the evolution of the linkage po wer in the course of panel reduction were determined. Guidelines for constr ucting a WGRH panel are proposed.