Serotype 14 variants of the Spanish penicillin-resistant serotype 9V cloneof Streptococcus pneumoniae arose by large recombinational replacements ofthe cpsA-pbp1a region

The high prevalence of penicillin resistance among Streptococcus pneumoniae isolates from Uruguay has been associated with the emergence of a penicill in-resistant clone of serotype 14. Isolates of this clone were identical by multilocus sequence typing to members of the Spanish penicillin-resistant serotype 9V clone and possessed indistinguishable forms of the penicillin-b inding protein 2b and 2x genes. Their pbp1a genes were also identical, exce pt at the 3' end. The serotype 14 isolates were shown to be a variant of th e Spanish serotype 9V clone which arose by a 22.2 kb recombinational replac ement that introduced the capsular biosynthetic locus, and part of the neig hbouring pbp1a gene, from a serotype 14 isolate. One end of the recombinati onal replacement was within the first gene of the capsular polysaccharide o peron, cpsA, as the sequence of the upstream dexB gene, through most of cps A, was identical in the penicillin-resistant serotype 9V and 14 isolates, b ut the sequences differed in the rest of cpsA and in cpsB. The other recomb inational junction was at the end of the divergently transcribed pbp1a gene , which is approximately 11.6 kb downstream of the capsular biosynthetic lo cus. Isolates of this serotype variant were also detected in Spain and Denm ark. Penicillin-resistant serotype 14 isolates from Poland were also closel y related to the penicillin-resistant serotype 9V clone, but have emerged i ndependently, as one end of the recombinational replacement was upstream of dexB and the other was within pbp1a, but at a different position from that in the serotype 14 variants from Uruguay, Spain and Denmark. Serotype 14 v ariants of the Spanish serotype 9V clone have therefore arisen on more than one occasion by large recombinational replacements that extend from the st art of the cps region into the pbp1a gene.