A rapid method for detecting and distinguishing clinically important yeasts by Heteroduplex Mobility Assays (HMAs)

Authors
Olicio, R Almeida, CAS Seuanez, HN
Citation
R. Olicio et al., A rapid method for detecting and distinguishing clinically important yeasts by Heteroduplex Mobility Assays (HMAs), MOL CELL PR, 13(4), 1999, pp. 251-255
Citations number
30
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR AND CELLULAR PROBES
ISSN journal
08908508 → ACNP
Volume
13
Issue
4
Year of publication
1999
Pages
251 - 255
Database
ISI
SICI code
0890-8508(199908)13:4<251:ARMFDA>2.0.ZU;2-B
Abstract
Heteroduplex Mobility Assays (HMAs) of 700 bp amplified products of a 17S r DNA region were used to identify and differentiate seven yeast species of c linical importance: Candida albicans, Torulopsis (Candida) glabrata, Candid a tropicalis, Candida parapsilosis, Candida (Pichia) guilliermondii and Han senula (Pichia) anomala. Distance of heteroduplex migration (dHE) was found to be negatively correlated to the number of nucleotide differences betwee n amplified DNA sequences. (C) 1999 Academic Press.