Ames assays and unscheduled DNA synthesis assays on 2,4-dichlorophenoxyacetic acid and its derivatives

2,4-Dichlorophenoxyacetic acid and several of its derivatives (collectively known as 2,4-D) are herbicides used to control a wide variety of broadleaf and woody plants. The genetic toxicity in vitro of 2,4-D and seven of its salts and esters were examined by employing gene mutation in bacteria (Ames test) and induction of DNA damage and repair in rat hepatocytes. In additi on, an in vivo unscheduled DNA synthesis (UDS) assay was performed on 2,4-D . There were no indications of genotoxic potential for 2,4-D acid, or any o f its derivatives, in these assays. These results are consistent with the r eported lack of carcinogenic potential for 2,4-D in both mice and rats. (C) 1999 Elsevier Science B.V. All rights reserved.