The restorer Rfo gene acts post-translationally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissues of rapeseed cybrids
M. Bellaoui et al., The restorer Rfo gene acts post-translationally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissues of rapeseed cybrids, PLANT MOL B, 40(5), 1999, pp. 893-902
This paper describes the analysis of the effect of the restorer gene Rfo on
the expression of the ORF138 protein associated with Ogura cytoplasmic mal
e sterility (CMS) which has been engineered in rapeseed by protoplast fusio
n. We show that the presence of the Rfo gene in the genome of the plants de
creases the amount of ORF138 protein in floral buds, this effect being the
most dramatic in anthers at the stage of development when the sterile pheno
type is normally expressed. However, the amount of orf138 transcripts is no
t affected by the Rfo gene in the same organs at the same stages. Total pol
ysome analyses of buds and anthers show that the orf138 transcripts are tra
nslated with the same efficiency in sterile and restored plants. From these
results we infer that the Rfo gene product acts on the post-translational
stability of the ORF138 protein, leading to a decrease in the accumulation
of the protein and a restoration of fertility.