The free energies for mutating S27 and W79 to alanine in streptavidin and its biotin complex: The relative size of polar and nonpolar free energies on biotin binding

Authors
Dixon, RW Kollman, P
Citation
Rw. Dixon et P. Kollman, The free energies for mutating S27 and W79 to alanine in streptavidin and its biotin complex: The relative size of polar and nonpolar free energies on biotin binding, PROTEINS, 36(4), 1999, pp. 471-473
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PROTEINS-STRUCTURE FUNCTION AND GENETICS
ISSN journal
08873585 → ACNP
Volume
36
Issue
4
Year of publication
1999
Pages
471 - 473
Database
ISI
SICI code
0887-3585(19990901)36:4<471:TFEFMS>2.0.ZU;2-Z
Abstract
We have carried out calculations on the relative free energy of binding of biotin and its S27A and W79A mutants to streptavidin, Consistent with earli er suggestions by Miyamoto and Kollman from free energy component analysis and recent experiments by Stayton and coworkers, the reduction in binding s trength by the W79A mutant is significantly larger than that of the S27A mu tant. Proteins 1999;38:471-473, (C) 1999 Wiley-Liss, Inc.