The free energies for mutating S27 and W79 to alanine in streptavidin and its biotin complex: The relative size of polar and nonpolar free energies on biotin binding
Rw. Dixon et P. Kollman, The free energies for mutating S27 and W79 to alanine in streptavidin and its biotin complex: The relative size of polar and nonpolar free energies on biotin binding, PROTEINS, 36(4), 1999, pp. 471-473
We have carried out calculations on the relative free energy of binding of
biotin and its S27A and W79A mutants to streptavidin, Consistent with earli
er suggestions by Miyamoto and Kollman from free energy component analysis
and recent experiments by Stayton and coworkers, the reduction in binding s
trength by the W79A mutant is significantly larger than that of the S27A mu
tant. Proteins 1999;38:471-473, (C) 1999 Wiley-Liss, Inc.