The diagnosis of freemartinism in cattle using sex-specific DNA sequences

Authors
Ennis, S Vaughan, L Gallagher, TF
Citation
S. Ennis et al., The diagnosis of freemartinism in cattle using sex-specific DNA sequences, RES VET SCI, 67(1), 1999, pp. 111-112
Citations number
20
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
RESEARCH IN VETERINARY SCIENCE
ISSN journal
00345288 → ACNP
Volume
67
Issue
1
Year of publication
1999
Pages
111 - 112
Database
ISI
SICI code
0034-5288(199908)67:1<111:TDOFIC>2.0.ZU;2-O
Abstract
The majority of heifers born co-twins to bulls are infertile freemartins. I t is important that the condition be diagnosed at an early age as freemarti ns have no potential for use as replacement stock. A rapid, robust, reliabl e technique for freemartin diagnosis is described. Three Y-specitic polymer ase chain reaction (PCR) primer pairs: BOV97M, BRY.1 and AMX/Y were used to detect male cells in the blood of heifers born co-twins to bulls. Pea-base d tests have advantages over currently used methods of freemartin diagnosis .