Seed storage protein characterization of Solanum species and of cultivars and androgenetic lines of S-melongena L. by SDS-PAGE and AE-HPLC

Authors
Mennella, G Sanaja', VO Tonini, A Magnifico, V
Citation
G. Mennella et al., Seed storage protein characterization of Solanum species and of cultivars and androgenetic lines of S-melongena L. by SDS-PAGE and AE-HPLC, SEED SCI T, 27(1), 1999, pp. 23-35
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
SEED SCIENCE AND TECHNOLOGY
ISSN journal
02510952 → ACNP
Volume
27
Issue
1
Year of publication
1999
Pages
23 - 35
Database
ISI
SICI code
0251-0952(1999)27:1<23:SSPCOS>2.0.ZU;2-D
Abstract
6 Solanum species, 20 S. melongena L. cultivars and 21 double-haploid andro genetic lines were characterized by seed storage protein analysis. Anionic exchange High Performance Liquid Chromatography (AE-HPLC) elution profiles and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) b anding patterns from either water- or basic-soluble proteins demonstrated d egrees of polymorphism. Solanum species and S.,melongena cultivars were cle arly identified by both methods, although with a different level of resolut ion. The androgenetic lines were divided into six groups by SDS-PAGE analys is whereas AE-HPLC allowed the distinction between lines belonging to the s ame group. A combination of both methods enabled the biochemical characteri zation of the tested samples. Different growth and environmental conditions did not influence the composition of Solanum alkali-soluble seed protein e xtracts.