Cell-surface expression of heat shock proteins in dog neutrophils after oxidative stress

Citation
A. Camins et al., Cell-surface expression of heat shock proteins in dog neutrophils after oxidative stress, TOX VITRO, 13(3), 1999, pp. 437-443
Citations number
36
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICOLOGY IN VITRO
ISSN journal
08872333 → ACNP
Volume
13
Issue
3
Year of publication
1999
Pages
437 - 443
Database
ISI
SICI code
0887-2333(199906)13:3<437:CEOHSP>2.0.ZU;2-I
Abstract
The effect of oxidative stress induced by different concentrations of hydro gen peroxide on dog neutrophils was studied. This effect was measured using dichlorofluorescein-diacetate (DCFH-DA) and by the cell surface membrane e xpression of heat shock protein (HSP) 27 kDa, HSP 72 kDa and HSP 90 kDa fam ilies. Hydrogen peroxide induced a concentration-dependent increase in DCFH oxidation (from 10(-6) M to 10(-4) M), and an increase in the cell surface expression of HSPs families. At a concentration of 10-4 hi, the percentage of positive cells that showed an oxidation of DCFH was 94.7% +/- 5.2 (n = 3). Only vitamin E (but not vitamin C) at a concentration of 0.5 mM was abl e to inhibit the intracellular oxidative stress induced by hydrogen peroxid e. The percentage of positive cells that express these proteins after the t reatment with hydrogen peroxide (10-4 M) was: 74% +/- 3.5 for HSP 27, 72% /- 2.6 for HSP 72 and 73% +/- 1.2 for HSP 90 (n = 3). This cell surface exp ression was not abolished by either vitamin C or vitamin E. Localization of HSPs in plasma membrane is of immunological interest because they have bee n implicated in autoimmune diseases. (C) 1999 Elsevier Science Ltd. All rig hts reserved.