Md. Edington et al., FEMTOSECOND TRANSIENT HOLE-BURNING DETECTION OF INTEREXCITON-STATE RADIATIONLESS DECAY IN ALLOPHYCOCYANIN TRIMERS, JOURNAL OF PHYSICAL CHEMISTRY B, 101(22), 1997, pp. 4473-4477
In its trimeric aggregation state, the phycobiliprotein allophycocyani
n contains the simplest possible system, an isolated pair of chromopho
res, in which delocalized excited states can be studied. By moving the
pump wavelength across allophycocyanin's ground-state absorption band
, we have obtained femtosecond time-resolved pump-probe spectra that e
vidence interexciton-state radiationless decay from the upper exciton
state and vibrational relaxation in the lower exciton state. Direct ev
idence for interexciton-state radiationless decay is taken from the in
itial appearance of photobleaching/stimulated-emission holes displaced
to the red with respect to the pump-pulse spectrum. The holes broaden
on the 120-fs time scale owing to intramolecular vibrational redistri
bution and transient solvation and shift to the red on the 230-fs time
scale owing to vibrational equilibration and transient solvation. We
show that vibrational relaxation in the lower exciton state contribute
s to the decay of a shoulder in the 590-640-nm region on the 400-fs ti
me scale when 620-nm excitation is employed but not when the pump spec
trum is tuned farther to the red, away from the 0 --> 1 vibronic trans
ition to the lower exciton state. In the absence of vibrational relaxa
tion in the lower exciton state, we can now discern that exciton local
ization contributes to the time evolution in the 590-640-nm region of
the spectrum on the 1-ps time scale.