Crystallization and preliminary crystallographic study of HIP/PAP, a humanC-lectin overexpressed in primary liver cancers

Authors
Abergel, C Chenivesse, S Stinnakre, MG Guasco, S Brechot, C Claverie, JM Devinoy, E Christa, L
Citation
C. Abergel et al., Crystallization and preliminary crystallographic study of HIP/PAP, a humanC-lectin overexpressed in primary liver cancers, ACT CRYST D, 55, 1999, pp. 1487-1489
Citations number
20
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
8
Pages
1487 - 1489
Database
ISI
SICI code
0907-4449(199908)55:<1487:CAPCSO>2.0.ZU;2-#
Abstract
Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Pan eth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crysta llized using the Hampton Research Crystal Screen and SAmBA software to defi ne the optimal crystallization protocol. The crystals belong to the orthorh ombic space group P2(1)2(1)2(1), With unit-cell parameters a = 30.73, b = 4 9.35, c = 92.15 Angstrom and one molecule in the asymmetric unit. Flash-fro zen crystals diffract to 1.78 Angstrom resolution using synchrotron. radiat ion. A molecular-replacement solution was obtained using the human Reg/ lit hostathine structure and the AMoRe software.