Fluorimetric method for the determination of thiocyanate with 2 ',7 '-dichlorofluorescein and iodine

Authors
Gong, BL Gong, GQ
Citation
Bl. Gong et Gq. Gong, Fluorimetric method for the determination of thiocyanate with 2 ',7 '-dichlorofluorescein and iodine, ANALYT CHIM, 394(2-3), 1999, pp. 171-175
Citations number
14
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
00032670 → ACNP
Volume
394
Issue
2-3
Year of publication
1999
Pages
171 - 175
Database
ISI
SICI code
0003-2670(19990809)394:2-3<171:FMFTDO>2.0.ZU;2-F
Abstract
A highly sensitive and selective fluorimetric method has been developed for the determination of thiocyanate with 2',7'-dichlorofluorescein (lambda(ex ) = 508 nm, lambda(em) = 524 nm) at pH 5.8-6.7. The calibration gaph is lin ear over the range 1.0-60 ng ml(-1) SCN-. The detection limit is 1.0 ng ml( -1) SCN-. The method has been successfully applied to the determination of thiocyanate ion in saliva and serum. (C) 1999 Elsevier Science B.V. All rig hts reserved.