A disposable creatinine sensor has been developed using a thick-film carbon
electrode for hydrogen peroxide. Carbon paste containing 10% Pt powder was
metal mask printed on a plastic plate, and irradiated by UV Light under pu
re oxygen atmosphere for activation of thick-film electrodes. Thin layer of
three different enzymes involving creatininase, creatinase, and sarcosine
oxidase was fabricated beside the printed electrode to form a creatinine se
nsor. A drop of a test solution automatically runs through a microchannel r
eaching the enzyme layer and then the enzymes quickly become solubilized an
d disperse throughout the volume of the microchannel. The detection of hydr
ogen peroxide at 500 mV vs. Ag/AgCl serves as the analytical signal. The se
nsor shows a linear response range for creatinine between 0.2 and 2 mM in a
pH 7.5 buffered solution with 0.1 M phosphate and 0.1 M KCl. The oxygen pl
asma treatment was found effective on improving the voltammetric behavior o
f the thick-film electrodes. (C) 1999 Elsevier Science B.V. All rights rese
rved.