Characterization of the human SDHD gene encoding the small subunit of cytochrome b (cybS) in mitochondrial succinate-ubiquinone oxidoreductase

Citation
H. Hirawake et al., Characterization of the human SDHD gene encoding the small subunit of cytochrome b (cybS) in mitochondrial succinate-ubiquinone oxidoreductase, BBA-BIOENER, 1412(3), 1999, pp. 295-300
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN journal
00052728 → ACNP
Volume
1412
Issue
3
Year of publication
1999
Pages
295 - 300
Database
ISI
SICI code
0005-2728(19990804)1412:3<295:COTHSG>2.0.ZU;2-4
Abstract
We have mapped large (cybL) and small (cybS) subunits of cytochrome b in th e succinate-ubiquinone oxidoreductase (complex II) of human mitochondria to chromosome 1q21 and 11q23, respectively (H. Hirawake et al., Cytogenet. Ce ll Genet. 79 (1997) 132-138). In the present study, the human SDHD gene enc oding cybS was cloned and characterized. The gene comprises four exons and three introns extending over 19 kb. Sequence analysis of the 5' promoter re gion showed several motifs for the binding of transcription factors includi ng nuclear respiratory factors NRF-1 and NRF-2 at positions -137 and -104, respectively. In addition to this gene, six pseudogenes of cybS were isolat ed and mapped on the chromosome. (C) 1999 Elsevier Science B.V. All rights reserved.