For designing the large scale protease production system, through solid sta
te fermentation, the important parameters are accumulation density, bed hei
ght, aeration etc. The optimum fermentation condition recorded were 0.3125
g/cc accumulation density and 1.0 cm bed height (without aeration). Aeratio
n at 3 LPM and 2.0 cm bed height were found optimum for protease production
by Rhizopus oryzae.