Production of ethanol by raw starch hydrolysis and fermentation of damagedgrains of wheat and sorghum

The simultaneous saccharification and fermentation was used to produce etha nol from raw starch of damaged quality wheat and sorghum grains by utilisin g crude amylase preparation from B. subtilis VB2 and an amylolytic yeast st rain S. cerevisiae VSJ4. Various concentrations of damaged wheat and sorghu m starch from 10% to 30%W/V were used and 25% was found to be optimum for d amaged wheat and sorghum starch yielding 4.40%V/V and 3.50%V/V ethanol resp ectively. Whereas 25% raw starch of fine quality wheat and sorghum grains g ave an yield of 5.60%V/V and 5.00%V/V respectively. The process was carried out at 35 degrees C, 5.8 pH and 200 rpm for 4 days.