Complete remission in acute myeloid leukaemia with t(8;21) following treatment with G-CSF: flow cytometric analysis of in vivo and in vitro effects on cell maturation

A 75-year-old patient diagnosed as having acute myeloid leukaemia with t(8; 21) received G-CSF alone as induction therapy. Complete remission was achie ved following 2 weeks of treatment. Flow cytometric analysis, performed by CD45 technique modified by the introduction of preliminary gating with LDS- 751, confirmed the disappearance of blast cells along with myeloid maturati on. Finally, in vitro studies demonstrated that G-CSF as compared to other differentiation inducers, was able to induce a striking effect toward neutr ophilic differentiation.