High - performance liquid chromatography method for determination of nitren
dipine in human serum is presented. The separation was achieved on ODS colu
mn, the mobile phase was methanol-water. UV detection was applied. Nifedipi
ne was used as an internal standard. The drugs were extracted from serum an
d saliva with diethyl ether. The detection limit was 2 ng ml(-1). The linea
rity of detection was good for the concentration range 10-60 ng ml(-1). The
method was applied for the monitoring of nitrendipine in patients serum af
ter oral administration and in serum and saliva after sublingual administra
tion.