Spectrophotometric determination of zinc with phenylfluorone in the presence of triton X-100 and cetylpyridinium chloride

Authors
Winkler, W Arenhovel-Pacula, A
Citation
W. Winkler et A. Arenhovel-pacula, Spectrophotometric determination of zinc with phenylfluorone in the presence of triton X-100 and cetylpyridinium chloride, CHEM ANAL, 44(4), 1999, pp. 725-730
Citations number
29
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHEMIA ANALITYCZNA
ISSN journal
00092223 → ACNP
Volume
44
Issue
4
Year of publication
1999
Pages
725 - 730
Database
ISI
SICI code
0009-2223(1999)44:4<725:SDOZWP>2.0.ZU;2-N
Abstract
Spectrophotometric determination of zinc using the reaction with phenylfluo rone (PF) in the presence of cetylpyridinium chloride (CP) and Triton X-100 was elaborated. Optimum concentrations of PF, CP and Triton X-100, and pH ensuring maximum absorbance were defined. The effect of foreign ions was el ucidated. The maximum absorbance is observed at 573 nm with the molar absor ptivity 1.09x10(5) l mol(-1) cm(-1). The Beer's law is obeyed for zinc conc entrations in the range 0.04-0.4 mu g ml(-1). The method has been applied f or determination of zinc in insulin.