Label of AFP antibody and electrochemiluminescence immunoassay

A new chemiluminescent reagent isothiocyanato-isoluminol (ITCI) was used to label Anti-AFP, The molar incorporation ratios are estimated to be 0.25 si milar to 0.50 mole of ITCI per mole Anti-AFP, The results show that there a re no apparent changes in the immunoreactivity of the labeled Anti-AFP and the quantum efficiency of ITCI attached to the Anti-AFP, In addition, the c onditions of labeling reaction, sandwich immunoassay and electro-chemilumin escent reaction were studied. Morever, a new sandwich electrochemiluminesce nce immunoassay(ECLIA) is firstly established, and used to detect AFP in hu man serum. The calibration ranges is 5.0 similar to 100.0 ng/mL and the min imal detection concentration is 2.0 ng/ mL.