Determination of ascorbic acid in plasma and urine by high performance liquid chromatography with ultraviolet detection

A reliable simple reversed-phase liquid chromatographic method for the rout ine determination of ascorbic acid in plasma and urine with ultraviolet det ection is described. This method enables the complete separation of the asc orbic acid peak from others with a recovery of above 95 % within 8 minutes. The method can be used for analysing multiple samples within a day. In add ition, the storage conditions and stability of ascorbic acid in plasma and urine were investigated. Samples of plasma and urine can be stored on ice i n darkness for at least 60 min without reduction of ascorbic acid concentra tion. Prepared samples can be stored in darkness at 4 degrees C for at leas t 120 min and in liquid nitrogen for 42 days.