Genomic structure and chromosome location of RPL27A/Rpl27a, the genes encoding human and mouse ribosomal protein L27A

The intron-containing genes encoding human and mouse ribosomal protein (r-p rotein) L27A were cloned and sequenced, The human r-protein L27A gene (RPL2 7A) shared an identical exon/intron structure with the mouse r-protein 27A gene (Rpl27a). The translational start codon ATG was separated from the mai n reading frame by the first intron sequence in both genes. An approximatel y 200-bp sequence upstream of the translational start site of both genes di splayed remarkable similarity, and contained the putative promoters lacking canonical TATA, but harbored Spl binding sites and a short stretch of pyri midine cluster, similar to other r-protein genes. Transcriptional regulator y elements, Box-A and GABP, found in the promoters of some other r-protein genes were also conserved in both genes. These structural features were inc luded in the typical CpG island identified in the 5'-end sequences, suggest ing that RPL27A/Rpl27a cloned here are authentic and transcriptionally acti ve. Fluorescence in situ hybridization (FISH) analysis localized the mouse intron-containing Rpl27a to chromosome 7E2-F1 syntenic to human chromosome 11P15, where human RPL27A was located.