The TGF-beta 2 isoform is both a required and sufficient inducer of murinehair follicle morphogenesis

Hair follicle development serves as an excellent model to study control of organ morphogenesis. Three specific isoforms of TGP-beta exist which exhibi t a distinct pattern of expression during hair follicle morphogenesis. To c larify the still elusive role of these factors in hair follicle development , we have used a combined genetic and functional approach: analysis of hair follicle development in mice with disruptions of the TGF-beta 1, 2,and 3 g enes was coupled with a direct functional test of the effect of added purif ied factors on fetal hair follicle development in skin organ cultures. TGF- beta 2 null mice exhibited a profound delay of hair follicle morphogenesis, with a 50% reduced number of hair follicles, In contrast to hair follicle development, growth and differentiation of interfollicular keratinocytes pr oceeded unimpaired. Unlike TGF-beta 2-/- mice, mice with a disruption of th e TGF-beta 1 gene showed slightly advanced hair follicle formation, while l ack of the TGF-beta 3 gene did not have any effects. Treatment of wild-type , embryonic skin explants (E14.5 or E15.5) with TGF-beta 2 protein in eithe r soluble form or slow release beads induced hair follicle development and epidermal hyperplasia, while similar TGP-beta 1 treatment exerted suppressi ve effects. Thus, the TGP-beta 2 isoform plays a specific role, not shared by the other TGF-beta isoforms, as an inducer of hair follicle morphogenesi s and is both required and sufficient to promote this process. (C) 1999 Aca demic Press.