Cellulase production by Neurospora crassa on wheat straw

Citation
Md. Romero et al., Cellulase production by Neurospora crassa on wheat straw, ENZYME MICR, 25(3-5), 1999, pp. 244-250
Citations number
17
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN journal
01410229 → ACNP
Volume
25
Issue
3-5
Year of publication
1999
Pages
244 - 250
Database
ISI
SICI code
0141-0229(199908)25:3-5<244:CPBNCO>2.0.ZU;2-Z
Abstract
The production of enzymes of the cellulase complex by the fungus Neurospora crassa on milled and sieved wheat straw was studied. The effects of straw concentration, temperature, and initial pH on the production of the beta-gl ucosidase, exoglucanase, and endoglucanase activities, as well as extracell ular and mycelial protein, were observed. With an initial pH value of 6.5 a nd a temperature of 30 degrees C, a straw concentration of 5% yielded the h igher activities for all the enzymes. Two maxima were observed both for bet a-glucosidase and endoglucanase, while the exoglucanase activity was consta nt from the end of the exponential phase. When a protease inhibitor was add ed, the production of beta-glucosidase was increasing without fluctuations. When the temperature was 25 degrees C, the enzymes production was lower, w hereas the mycelial protein value was higher. Changes in the initial pH, lo wering or raising it, lowered the enzymes production, but favored fungus gr owth. (C) 1999 Elsevier Science Inc. All rights reserved.