Induction of anthocyanin synthesis in nonpigmented grape cell suspensions by acting on DFR substrate availability or precursors level

Teinturier variety grape cell cultures produce anthocyanins and exhibit a r ed appearance, although the major cell population is composed of nonpigment ed cells. A novel cell suspension culture was initiated from selected nonpi gmented cells that were shown, to synthesize catechins. Anthocyanin synthes is has been triggered when cell division was reduced by Pi deficiency. Furt hermore, the addition of dihydroquercetin resulted in a 10-fold increase in dihydroflavonol 4-reductase (DFR) activity. Tn nonpigmented cells a higher level of precursors or an elevated level of substrate availability would i nduce anthocyanin synthesis. (C) 1999 Elsevier Science Inc. All rights rese rved.