Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques

Citation
T. Lehner et al., Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques, EUR J IMMUN, 29(8), 1999, pp. 2427-2435
Citations number
38
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
00142980 → ACNP
Volume
29
Issue
8
Year of publication
1999
Pages
2427 - 2435
Database
ISI
SICI code
0014-2980(199908)29:8<2427:IOIATT>2.0.ZU;2-3
Abstract
The seven-transmembrane G-protein-linked CCR5 molecule functions as a major coreceptor for HIV or simian immunodeficiency virus (SIV) infection. Antib odies to CCR5 were studied in rhesus macaques immunized with SIV grown in h uman CD4(+) T cells. These macaques were completely protected against i.v. challenge with live SIV. Sera from the protected macaques showed significan tly greater inhibition of SIV replication (p < 0.001) and macrophage inflam matory protein-1 beta-generated CCR5-dependent chemotaxis (p < 0.01) than s era from unprotected macaques, in the absence of significant neutralizing a ntibodies to SIV. These two functional assays demonstrate serum antibodies to the CCR5 receptors which were specifically inhibited by CCR5-transfected HEK-293 cells. We postulate that anti-CCR5 antibodies may be complementary to beta-chemokines in blocking CCR5 coreceptors to HIV or SIV binding and fusion of CD4(+) cells.