Sh. Inayat-hussain et al., Caspases-3 and-7 are activated in goniothalamin-induced apoptosis in humanJurkat T-cells, FEBS LETTER, 456(3), 1999, pp. 379-383
Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus
andersonii, induced apoptosis in Jurkat T-cells as assessed by the external
isation of phosphatidylserine. Immunoblotting showed processing of caspases
-3 and -7 with the appearance of their catalytically active large subunits
of 17 and 19 kDa, respectively. Activation of these caspases mas further ev
idenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-tr
eatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluorometh
yl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these
caspases and PARP, Our results demonstrate that activation of at least two
effector caspases is a key feature of goniothalamin-induced apoptosis in J
urkat T-cells, (C) 1999 Federation of European Biochemical Societies.