Effect of the in vivo catalase inhibition on aminonucleoside nephrosis

Reactive oxygen species have been involved in the pathophysiology of puromy cin aminonucleoside (PAN)-nephrosis. The role of H2O2 in these rats may be studied modulating the amount or activity of catalase, which breakdowns H2O 2 to water and oxygen. To explore the role of H2O2 in this experimental mod el, we studied the effect of the in vivo catalase inhibiton with 3-amino-1, :2,4-triazole (ATZ) on the course of PAN-nephrosis. Four groups of rats wer e studied: control rats (CT group), PAN-injected rats (PAN group), ATZ-inje cted rats (ATZ group), and ATZ- and PAN-injected rats (ATZPAN group). Rats were placed in metabolic cages to collect 24 h urine along the study, ATZ ( 1 g/kg) was given 24 h before PAN injection (75 mg/kg), and the proteinuria was measured on days 0, 2, 4, 6, 8, and 10. Proteinuria started before (da y 4) and was significantly higher on days 6, 8, and 10 in the ATZPAN group than in the PAN group. On day 10, hypercholesterolemia was significantly hi gher in the ATZPAN group than in the PAN group. These data indicate that th e in vivo catalase inhibition magnifies PAN-nephrosis, suggesting that H2O2 is produced in vivo and involved in the renal damage in this experimental disease. (C) 1999 Elsevier Science Inc.