Isolation and characterization of simple sequence repeat loci in the gray tree frog, Hyla chrysoscelis

A gray tree frog (Hyla chrysoscelis) genomic library was constructed and ch aracterized with regard to the incidence and complexity of simple sequence repeat (SSR) loci. The partial genomic library, containing approximately 10 000 clones with an average-sized insert of 350 bp, was screened with six S SR repeat oligonucleotides (AC, AG, ACG, AGC, AAC, and AAG). Screening iden tified 31 unique positive clones containing 41 SSR loci. Sequences of tande mly arrayed dinucleotide repeats were more common (36 of 41) than trinucleo tide repeats. Twenty-six loci were identified using the AC dinucleotide pro be, while 7 loci were identified using the AG dinucleotide probe. An additi onal 3 AT dinucleotide loci were serendipitously identified. The AT repeats generally comprised the longest dinucleotide repeat loci. The SSR repeat l oci reported here should provide potent markers for identity, parentage, an d short-lineage determinations in large-scale experiments using gray tree f rogs.