A. Leonowicz et al., Cooperation of fungal laccase and glucose 1-oxidase in transformation of Bjorkman lignin and some phenolic compounds, HOLZFORSCH, 53(4), 1999, pp. 376-380
A screening of wood-rotting basidiomycete fungi was conducted for glucose l
-oxidase (GOD) and laccase (LAC) production as well as for ligninolytic act
ivity measured by a Rhemazol reaction. The results showed that genera rich
in GOD are lignin degraders as well as effective producers of extracellular
LAG. The fungi poor in GOD neither showed LAG, nor ligninolytic activity.
The Bjorkman lignin and 3 phenolic compounds, hydroquinone and syringic and
vanillic acids, were tested on the sequential activity of LAC and GOD. In
the presence of LAG, quinoid intermediates formed from Bjorkman lignin and
phenolic compounds were observed. The addition of GOD caused a diminution o
f the quinone level. During incubation of Bjorkman lignin with LAC and GOD
depolymerization occurred, and in the experiments omitting GOD the quantiti
es of low molecular products were markedly lower. Consequently, the consecu
tive ping-pong activity of LAC and GOD reduced the polymerization and impro
ved the efficiency of depolymerization processes.