Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins

The deduced sequences of the Omp10, Omp16, and Omp19 outer membrane protein s of Brucella spp. contain a potential bacterial lipoprotein processing seq uence. After extraction with Triton X-114, these three proteins partitioned into the detergent phase. Processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. The thre e proteins were radioimmunoprecipitated from [H-3]palmitic acid-labeled Bru cella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins.