ISOLATION OF A GENE ENCODING A NOVEL SPECTINOMYCIN PHOSPHOTRANSFERASEFROM LEGIONELLA-PNEUMOPHILA

Authors
SUTER TM VISWANATHAN VK CIANCIOTTO NP
Citation
Tm. Suter et al., ISOLATION OF A GENE ENCODING A NOVEL SPECTINOMYCIN PHOSPHOTRANSFERASEFROM LEGIONELLA-PNEUMOPHILA, Antimicrobial agents and chemotherapy, 41(6), 1997, pp. 1385-1388
Citations number
30
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
41
Issue
6
Year of publication
1997
Pages
1385 - 1388
Database
ISI
SICI code
0066-4804(1997)41:6<1385:IOAGEA>2.0.ZU;2-Y
Abstract
A gene capable of conferring spectinomycin resistance was isolated fro m Legionella pneumophila, the agent of Legionnaires' disease, The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases, Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demo nstrated resistance to spectinomycin, and Southern hybridizations dete rmined that nph also exists in other legionellae.